MCM2 Mouse Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: CF805620

Carrier-free (BSA/glycerol-free) MCM2 mouse monoclonal antibody, clone OTI3B6 (formerly 3B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (RC202506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2. Positive lysates LY401445 (100ug) and LC401445 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3B6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCM2 (NP_004517) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens minichromosome maintenance complex component 2 (MCM2), transcript variant 1, mRNA.
• Database Link:
  NP_004517
  Entrez Gene 17216 MouseEntrez Gene 312538 RatEntrez Gene 4171 Human
  UniProt ID P49736
• Synonyms: BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication