CANT1 Mouse Monoclonal Antibody [Clone ID: OTI7F8]

CAT#: CF805629

Carrier-free (BSA/glycerol-free) CANT1 mouse monoclonal antibody, clone OTI7F8 (formerly 7F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CANT1 (RC202348, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CANT1. Positive lysates LY408394 (100ug) and LC408394 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CANT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805629)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CANT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805629)

Specifications

Product Data

• Clone Name: OTI7F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 63-401 of human CANT1 (NP_620148) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.7 kDa
• Gene Name: Homo sapiens calcium activated nucleotidase 1 (CANT1), transcript variant 1, mRNA.
• Database Link:
  NP_620148
  Entrez Gene 76025 MouseEntrez Gene 246272 RatEntrez Gene 124583 Human
  UniProt ID Q8WVQ1
• Background: This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2010]
• Synonyms: DBQD; DBQD1; SCAN-1; SCAN1; SHAPY

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Purine metabolism, Pyrimidine metabolism