Rb (RB1) Mouse Monoclonal Antibody [Clone ID: OTI13A6]

CAT#: CF805640

Carrier-free (BSA/glycerol-free) RB1 mouse monoclonal antibody,clone OTI13A6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RB1 (RC206933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RB1 mouse monoclonal antibody.1:500`

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  Western blot analysis of extracts (35ug) from 4 different cell lines and 2 different tissue lysates by using anti-RB1 monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-RB1 monoclonal antibody. 1:400

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and RB1-Knockout Hela cells (KO, Cat# LC810199) were separated by SDS-PAGE and immunoblotted with anti-RB1 monoclonal antibody TA805640 (1:250). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805640) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805640) (1:500)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805640) (1:500)

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Specifications

Product Data

• Clone Name: OTI13A6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 309-590 of human RB1(NP_000312) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RB transcriptional corepressor 1
• Database Link:
  NP_000312
  Entrez Gene 19645 MouseEntrez Gene 24708 RatEntrez Gene 5925 Human
  UniProt ID P06400
• Synonyms: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer