ROS1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CAT#: CF805678

Carrier-free (BSA/glycerol-free) ROS1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (RC220652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using TA805678 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (TA805678; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (TA805678; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (TA805678; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (TA805678) (Left). The right is HELA cells as negative control (1:2000).

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Specifications

Product Data

• Clone Name: OTI1F3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:5000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2126-2347 of human ROS1(NP_002935) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ROS proto-oncogene 1, receptor tyrosine kinase (ROS1), mRNA.
• Database Link:
  NP_002935
  Entrez Gene 6098 Human
  UniProt ID P08922
• Background: This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
• Synonyms: c-ros-1; MCF3; ROS

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane