PROCR Mouse Monoclonal Antibody [Clone ID: OTI12H5]

CAT#: CF805770

Carrier-free (BSA/glycerol-free) PROCR mouse monoclonal antibody, clone OTI12H5 (formerly 12H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROCR (RC204858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROCR (1:2000). Positive lysates LY401925 (100ug) and LC401925 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805770) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805770) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805770) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805770) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805770) (1:150)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805770) (1:150)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805770) (1:150)

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Specifications

Product Data

• Clone Name: OTI12H5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-210 of human PROCR (NP_006395) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.7 kDa
• Gene Name: Homo sapiens protein C receptor (PROCR), mRNA.
• Database Link:
  NP_006395
  Entrez Gene 10544 Human
  UniProt ID Q9UNN8
• Background: The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. [provided by RefSeq, Jul 2013]
• Synonyms: CCCA; CCD41; EPCR

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane