MET Mouse Monoclonal Antibody [Clone ID: OTI3G5]

CAT#: CF805796

Carrier-free (BSA/glycerol-free) MET mouse monoclonal antibody, clone OTI3G5 (formerly 3G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MET (RC217003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MET. Positive lysates LY400094 (100ug) and LC400094 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded MET positive Human lung carcinoma tissue using anti-MET mouse monoclonal antibody. (TA805796) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI3G5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 570-885 of human MET(NP_000236) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant 2, mRNA.
• Database Link:
  NP_000236
  Entrez Gene 17295 MouseEntrez Gene 24553 RatEntrez Gene 4233 Human
  UniProt ID P08581
• Synonyms: AUTS9; c-Met; DFNB97; HGFR; RCCP2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma