hSET1 (SETD1A) Mouse Monoclonal Antibody [Clone ID: OTI7B7]

CAT#: CF805877

Carrier-free (BSA/glycerol-free) SETD1A mouse monoclonal antibody, clone OTI7B7 (formerly 7B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD1A (RC214996, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD1A. Positive lysates LY415047 (100ug) and LC415047 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SETD1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805877)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SETD1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805877)

Specifications

Product Data

• Clone Name: OTI7B7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-320 of human SETD1A(NP_055527) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 185.9 kDa
• Gene Name: Homo sapiens SET domain containing 1A, histone lysine methyltransferase (SETD1A), mRNA.
• Database Link:
  NP_055527
  Entrez Gene 233904 MouseEntrez Gene 9739 Human
  UniProt ID O15047
• Background: SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055). [supplied by OMIM, Mar 2008]
• Synonyms: KMT2F; Set1; Set1A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation