Factor I (CFI) Mouse Monoclonal Antibody [Clone ID: OTI4E9]

CAT#: CF805899

Carrier-free (BSA/glycerol-free) CFI mouse monoclonal antibody, clone OTI4E9 (formerly 4E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFI (RC216645, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFI. Positive lysates LY424865 (100ug) and LC424865 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4E9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 340-583 of human CFI(NP_000195) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.4 kDa
• Gene Name: Homo sapiens complement factor I (CFI), transcript variant 2, mRNA.
• Database Link:
  NP_000195
  Entrez Gene 3426 Human
  UniProt ID P05156
• Synonyms: AHUS3; ARMD13; C3b-INA; C3BINA; FI; IF; KAF

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades