SUOX Mouse Monoclonal Antibody [Clone ID: OTI5A2]

CAT#: CF805951

Carrier-free (BSA/glycerol-free) SUOX mouse monoclonal antibody, clone OTI5A2 (formerly 5A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUOX (RC209066, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUOX. Positive lysates LY424706 (100ug) and LC424706 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SUOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805951)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SUOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805951)

Specifications

Product Data

• Clone Name: OTI5A2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 320-545 of human SUOX(NP_000447) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.1 kDa
• Gene Name: Homo sapiens sulfite oxidase (SUOX), transcript variant 1, mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000447
  Entrez Gene 81805 RatEntrez Gene 211389 MouseEntrez Gene 6821 Human
  UniProt ID P51687
• Background: Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins. [provided by RefSeq, Jul 2008]
• Synonyms: OTTHUMP00000158619; sulfite oxidase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Sulfur metabolism