TFPI Mouse Monoclonal Antibody [Clone ID: OTI5H5]

CAT#: CF805999

Carrier-free (glycerol/BSA-free) TFPI mouse monoclonal antibody, clone OTI5H5 (formerly 5H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (RC219033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI. Positive lysates LY401895 (100ug) and LC401895 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TFPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805999)

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Specifications

Product Data

• Clone Name: OTI5H5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 29-304 of human TFPI (NP_006278) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.9 kDa
• Gene Name: Homo sapiens tissue factor pathway inhibitor (TFPI), transcript variant 1, mRNA.
• Database Link:
  NP_006278
  Entrez Gene 7035 Human
  UniProt ID P10646
• Synonyms: EPI; LACI; TFI; TFPI1

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Complement and coagulation cascades