Factor I (CFI) Mouse Monoclonal Antibody [Clone ID: OTI15E11]

CAT#: CF806033

Carrier-free (BSA/glycerol-free) CFI mouse monoclonal antibody, clone OTI15E11 (formerly 15E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFI (RC216645, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFI. Positive lysates LY424865 (100ug) and LC424865 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CFI mouse monoclonal antibody. (TA806033) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CFI mouse monoclonal antibody. (TA806033) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CFI mouse monoclonal antibody. (TA806033) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI15E11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 340-583 of human CFI(NP_000195) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.4 kDa
• Gene Name: Homo sapiens complement factor I (CFI), transcript variant 2, mRNA.
• Database Link:
  NP_000195
  Entrez Gene 3426 Human
  UniProt ID P05156
• Background: This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uraemic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immmune deposits is another condition associated with mutation of this gene. [provided by RefSeq, Jul 2008]
• Synonyms: AHUS3; ARMD13; C3b-INA; C3BINA; FI; IF; KAF

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades