NKRF Mouse Monoclonal Antibody [Clone ID: OTI4C2]

CAT#: CF806097

Carrier-free (BSA/glycerol-free) NKRF mouse monoclonal antibody, clone OTI4C2 (formerly 4C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKRF (RC208507, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKRF. Positive lysates LY402598 (100ug) and LC402598 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4C2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-296 of human NKRF(NP_060014) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.
• Database Link:
  NP_060014
  Entrez Gene 77286 MouseEntrez Gene 55922 Human
  UniProt ID O15226
• Synonyms: ITBA4; NRF

Reference Data

• Protein Families: Transcription Factors