THTPA Mouse Monoclonal Antibody [Clone ID: OTI4C11]

CAT#: CF806180

Carrier-free (BSA/glycerol-free) THTPA mouse monoclonal antibody, clone OTI4C11 (formerly 4C11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY THTPA (RC200849, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-THTPA. Positive lysates LY411308 (100ug) and LC411308 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4C11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-230 of human THTPA (NP_077304) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.4 kDa
• Gene Name: Homo sapiens thiamine triphosphatase (THTPA), transcript variant 1, mRNA.
• Database Link:
  NP_077304
  Entrez Gene 79178 Human
  UniProt ID Q9BU02
• Background: This gene encodes an enzyme which catalyzes the biosynthesis of thiamine disphophate (vitamin B1) by hydrolysis of thiamine triphosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
• Synonyms: THTP; THTPASE

Reference Data

• Protein Pathways: Metabolic pathways, Thiamine metabolism