Peripherin (PRPH) Mouse Monoclonal Antibody [Clone ID: OTI1E10]

CAT#: CF806183

Carrier-free (BSA/glycerol-free) PRPH mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPH (RC207561, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPH. Positive lysates LY401885 (100ug) and LC401885 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806183)

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Specifications

Product Data

• Clone Name: OTI1E10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRPH (NP_006253) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens peripherin (PRPH), mRNA.
• Database Link:
  NP_006253
  Entrez Gene 19132 MouseEntrez Gene 24688 RatEntrez Gene 5630 Human
  UniProt ID P41219
• Background: This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]
• Synonyms: NEF4; PRPH1

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)