Glucocorticoid Receptor (NR3C1) Mouse Monoclonal Antibody [Clone ID: OTI1B11]

CAT#: CF806197

Carrier-free (BSA/glycerol-free) NR3C1 mouse monoclonal antibody, clone OTI1B11 (formerly 1B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR3C1 (RC220189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR3C1 (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA806197) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806197) (1:500)

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Specifications

Product Data

• Clone Name: OTI1B11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-265 of human NR3C1(NP_000167) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens nuclear receptor subfamily 3 group C member 1 (NR3C1), transcript variant 1, mRNA.
• Database Link:
  NP_000167
  Entrez Gene 14815 MouseEntrez Gene 24413 RatEntrez Gene 2908 Human
  UniProt ID P04150
• Synonyms: GCCR; GCR; GCRST; GR; GRL

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Neuroactive ligand-receptor interaction