Angiopoietin 2 (ANGPT2) Mouse Monoclonal Antibody [Clone ID: OTI3H7]

CAT#: CF806213

Carrier-free (BSA/glycerol-free) ANGPT2 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANGPT2 (RC217216, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANGPT2. Positive lysates LY400459 (100ug) and LC400459 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ANGPT2 mouse monoclonal antibody. (TA806213; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ANGPT2 mouse monoclonal antibody. (TA806213; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Specifications

Product Data

• Clone Name: OTI3H7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-496 of human ANGPT2(NP_001138) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.9 kDa
• Gene Name: Homo sapiens angiopoietin 2 (ANGPT2), transcript variant 1, mRNA.
• Database Link:
  NP_001138
  Entrez Gene 11601 MouseEntrez Gene 89805 RatEntrez Gene 285 Human
  UniProt ID O15123
• Background: The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: AGPT2; ANG2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein