Glucocorticoid Receptor (NR3C1) Mouse Monoclonal Antibody [Clone ID: OTI6A12]

CAT#: CF806237

Carrier-free (BSA/glycerol-free) NR3C1 mouse monoclonal antibody, clone OTI6A12 (formerly 6A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR3C1 (RC220189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR3C1. Positive lysates LY424874 (100ug) and LC424874 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and NR3C1-Knockout Hela cells (KO, Cat# LC810096) were separated by SDS-PAGE and immunoblotted with anti-NR3C1 monoclonal antibody TA806237. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806237)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806237)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806237)

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Specifications

Product Data

• Clone Name: OTI6A12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-265 of human NR3C1(NP_000167) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.5 kDa
• Gene Name: Homo sapiens nuclear receptor subfamily 3 group C member 1 (NR3C1), transcript variant 1, mRNA.
• Database Link:
  NP_000167
  Entrez Gene 14815 MouseEntrez Gene 24413 RatEntrez Gene 2908 Human
  UniProt ID P04150
• Background: This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
• Synonyms: GCCR; GCR; GCRST; GR; GRL

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Neuroactive ligand-receptor interaction