NR2E3 Mouse Monoclonal Antibody [Clone ID: OTI2H9]

CAT#: CF806271

Carrier-free (BSA/glycerol-free) NR2E3 mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2E3 (RC220677, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR2E3. Positive lysates LY429495 (100ug) and LC429495 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2H9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NR2E3 (NP_057430) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens nuclear receptor subfamily 2 group E member 3 (NR2E3), transcript variant 1, mRNA.
• Database Link:
  NP_057430
  Entrez Gene 23958 MouseEntrez Gene 100365683 RatEntrez Gene 10002 Human
  UniProt ID Q9Y5X4
• Background: This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. Nuclear receptors have been shown to regulate pathways involved in embryonic development, as well as in maintenance of proper cell function in adults. Members of this family are characterized by discrete domains that function in DNA and ligand binding. This gene encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. Defects in this gene are a cause of enhanced S cone syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul
• Synonyms: ESCS; PNR; rd7; RNR; RP37

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors