NR0B2 Mouse Monoclonal Antibody [Clone ID: OTI8E5]

CAT#: CF806345

Carrier-free (BSA/glycerol-free) NR0B2 mouse monoclonal antibody, clone OTI8E5 (formerly 8E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2. Positive lysates LY402893 (100ug) and LC402893 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NR0B2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR0B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806345) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI8E5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NR0B2 (NP_068804) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens nuclear receptor subfamily 0 group B member 2 (NR0B2), mRNA.
• Database Link:
  NP_068804
  Entrez Gene 8431 Human
  UniProt ID Q15466
• Background: The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]
• Synonyms: SHP; SHP1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors