PCBP1 Mouse Monoclonal Antibody [Clone ID: OTI3D12]

CAT#: CF806353

Carrier-free (BSA/glycerol-free) PCBP1 mouse monoclonal antibody, clone OTI3D12 (formerly 3D12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCBP1 (RC207878, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCBP1. Positive lysates LY401867 (100ug) and LC401867 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines and 4 human tissue by using anti-PCBP1 monoclonal antibody (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806353)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806353)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806353)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806353)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806353)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806353)

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Specifications

Product Data

• Clone Name: OTI3D12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-225 of human PCBP1(NP_006187) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.
• Database Link:
  NP_006187
  Entrez Gene 23983 MouseEntrez Gene 500242 RatEntrez Gene 5093 Human
  UniProt ID Q15365
• Background: This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]
• Synonyms: HEL-S-85; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX

Reference Data

• Protein Pathways: Spliceosome