NR1D2 Mouse Monoclonal Antibody [Clone ID: OTI5E10]

CAT#: CF806365

Carrier-free (BSA/glycerol-free) NR1D2 mouse monoclonal antibody, clone OTI5E10 (formerly 5E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2. Positive lysates LY417483 (100ug) and LC417483 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5E10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-294 of human NR1D2(NP_005117) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64.5 kDa
• Gene Name: Homo sapiens nuclear receptor subfamily 1 group D member 2 (NR1D2), transcript variant 1, mRNA.
• Database Link:
  NP_005117
  Entrez Gene 259241 RatEntrez Gene 353187 MouseEntrez Gene 9975 Human
  UniProt ID Q14995
• Background: This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
• Synonyms: BD73; EAR-1R; RVR

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors