TNFRSF18 Mouse Monoclonal Antibody [Clone ID: OTI4A9]

CAT#: CF806458

Carrier-free (BSA/glycerol-free) TNFRSF18 mouse monoclonal antibody, clone OTI4A9 (formerly 4A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF18 (RC219997, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF18. Positive lysates LY418153 (100ug) and LC418153 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of human peripheral blood mononuclear cells (PBMCs) by using anti-TNFRSF18 monoclonal antibody. Left: Unstimulated PBMC. Right: PBMC stimulated with 10ug/ml Phytohemagglutinin for 3 days.

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  Flow cytometric analysis of TNFRSF18 expression on living stimulated human peripheral blood mononuclear cells (PBMCs). Unstimulated PBMCs, (a.left, b.blue), or 10ug/ml Phytohemagglutinin-stimulated (3 days) PBMCs, (a.right, b.red), were stained with Anti-Human TNFRSF18 antibody (TA806458) (1:100).

Specifications

Product Data

• Clone Name: OTI4A9
• Applications: FC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 26-162 of human TNFRSF18(NP_004186) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: Homo sapiens TNF receptor superfamily member 18 (TNFRSF18), transcript variant 1, mRNA.
• Database Link:
  NP_004186
  Entrez Gene 8784 Human
  UniProt ID Q9Y5U5
• Background: This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb
• Synonyms: AITR; CD357; GITR; GITR-D

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction