DCAMKL1 (DCLK1) Mouse Monoclonal Antibody [Clone ID: OTI1F8]

CAT#: CF806542

Carrier-free (BSA/glycerol-free) DCLK1 mouse monoclonal antibody,clone OTI1F8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLK1 (RC217050, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLK1 (1:2000). Positive lysates LY417785 (100ug) and LC417785 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-DCLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806542) (1:500).

Specifications

Product Data

• Clone Name: OTI1F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 193-492 of human DCLK1 (NP_004725) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.9 kDa
• Gene Name: Homo sapiens doublecortin like kinase 1 (DCLK1), transcript variant 1, mRNA.
• Database Link:
  NP_004725
  Entrez Gene 13175 MouseEntrez Gene 9201 Human
  UniProt ID O15075
• Synonyms: CL1; CLICK1; DCAMKL1; DCDC3A; DCLK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase