SCP3 (SYCP3) Mouse Monoclonal Antibody [Clone ID: OTI3D6]

CAT#: CF806547

Carrier-free (BSA/glycerol-free) SYCP3 mouse monoclonal antibody, clone OTI3D6 (formerly 3D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SYCP3 (RC209340, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYCP3. Positive lysates LY406978 (100ug) and LC406978 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3D6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SYCP3 (NP_710161) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.5 kDa
• Gene Name: synaptonemal complex protein 3
• Database Link:
  NP_710161
  Entrez Gene 50511 Human
  UniProt ID Q8IZU3
• Background: This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2010]
• Synonyms: COR1; SCP3; SPGF4