UBE2D2 Mouse Monoclonal Antibody [Clone ID: OTI1G4]

CAT#: CF806602

Carrier-free (BSA/glycerol-free) UBE2D2 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2. Positive lysates LY418757 (100ug) and LC418757 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1G4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UBE2D2 (NP_003330) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.6 kDa
• Gene Name: Homo sapiens ubiquitin conjugating enzyme E2 D2 (UBE2D2), transcript variant 1, mRNA.
• Database Link:
  NP_003330
  Entrez Gene 641452 RatEntrez Gene 7322 Human
  UniProt ID P62837
• Background: Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
• Synonyms: 5; E2(17)KB2; PUBC1; UBC4; UBCH4; UBCH5B

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis