UBE2D2 Mouse Monoclonal Antibody [Clone ID: OTI1G4]

CAT#: CF806602

Carrier-free (BSA/glycerol-free) UBE2D2 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)

Formulation: Standard Carrier Free


  View other "OTI1G4" antibodies (2)

USD 465.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI1G4
Applications WB
Recommended Dilution WB 1:2000
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human UBE2D2 (NP_003330) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 16.6 kDa
Gene Name Homo sapiens ubiquitin conjugating enzyme E2 D2 (UBE2D2), transcript variant 1, mRNA.
Background Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
Synonyms 5; E2(17)KB2; PUBC1; UBC4; UBCH4; UBCH5B
Reference Data
Protein Pathways Ubiquitin mediated proteolysis

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