Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: OTI2F2]

CAT#: CF806722

Carrier-free (BSA/glycerol-free) SPP1 mouse monoclonal antibody, clone OTI2F2 (formerly 2F2)

Formulation: Standard Carrier Free


  View other "OTI2F2" antibodies (2)

USD 465.00

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Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI2F2
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1(NP_001035147) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 33.7 kDa
Gene Name Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, mRNA.
Background The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Synonyms BNSP; BSPI; ETA-1; OPN
Reference Data
Protein Families Druggable Genome, Secreted Protein
Protein Pathways ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

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