CD252 (TNFSF4) Mouse Monoclonal Antibody [Clone ID: OTI5F10]

CAT#: CF806738

Carrier-free (BSA/glycerol-free) TNFSF4 mouse monoclonal antibody,clone OTI5F10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFSF4 (RC224021, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFSF. Positive lysates LY401143 (100ug) and LC401143 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5F10
• Applications: WB
• Recommended Dilution: WB 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TNFSF4 (NP_003317) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.9 kDa
• Gene Name: Homo sapiens TNF superfamily member 4 (TNFSF4), transcript variant 1, mRNA.
• Database Link:
  NP_003317
  Entrez Gene 7292 Human
  UniProt ID P23510
• Background: This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
• Synonyms: CD134L; CD252; GP34; OX-40L; OX4OL; TXGP1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction