FARSLB (FARSB) Mouse Monoclonal Antibody [Clone ID: OTI4B3]

CAT#: CF806739

Carrier-free (BSA/glycerol-free) FARSB mouse monoclonal antibody, clone OTI4B3 (formerly 4B3)

Product Images

Western blot analysis of extracts (10ug) from 5 different cell lines and 4 human tissue by using anti-FARSB monoclonal antibody (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus) at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-FARSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806739)

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-FARSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806739)

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  Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-FARSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806739)

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-FARSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806739)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-FARSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806739)

• Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-FARSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806739)

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Specifications

Product Data

• Clone Name: OTI4B3
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FARSB (NP_005678) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65.9 kDa
• Gene Name: Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant 1, mRNA.
• Database Link:
  NP_005678
  Entrez Gene 23874 MouseEntrez Gene 301544 RatEntrez Gene 10056 Human
  UniProt ID Q9NSD9
• Background: This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. [provided by RefSeq, Jul 2008]
• Synonyms: FARSLB; FRSB; HSPC173; PheHB; PheRS

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis