CCN2 Mouse Monoclonal Antibody [Clone ID: OTI5H7]

CAT#: CF806803

Carrier-free (BSA/glycerol-free) CTGF mouse monoclonal antibody, clone OTI5H7 (formerly 5H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTGF (RC210336, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTGF (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806803) (1:150)

Specifications

Product Data

• Clone Name: OTI5H7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 27-349 of human CTGF(NP_001892) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.9 kDa
• Gene Name: Homo sapiens cellular communication network factor 2 (CCN2), mRNA.
• Database Link:
  NP_001892
  Entrez Gene 1490 Human
  UniProt ID P29279
• Background: The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]
• Synonyms: CCN2; HCS24; IGFBP8; NOV2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein