FLT4 Mouse Monoclonal Antibody [Clone ID: OTI1G7]

CAT#: CF806894

Carrier-free (BSA/glycerol-free) FLT4 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLT4 (RC214285, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FLT4. Positive lysates LY405313 (100ug) and LC405313 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1G7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1063-1363 of human FLT4(NP_891555) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 150.2 kDa
• Gene Name: Homo sapiens fms related tyrosine kinase 4 (FLT4), transcript variant 1, mRNA.
• Database Link:
  NP_891555
  Entrez Gene 14257 MouseEntrez Gene 114110 RatEntrez Gene 2324 Human
  UniProt ID P35916
• Background: This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008]
• Synonyms: FLT-4; FLT41; LMPH1A; PCL; VEGFR-3; VEGFR3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion