Ezrin (EZR) Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CAT#: CF807019

Carrier-free (BSA/glycerol-free) EZR mouse monoclonal antibody,clone OTI1F3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EZR (RC200404, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EZR (1:500). Positive lysates LY401164 (100ug) and LC401164 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-EZR monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and EZR-Knockout HeLa cells (KO, Cat# LC810256) were separated by SDS-PAGE and immunoblotted with anti-EZR monoclonal antibody TA807019 (1:250). Then the blotted membrane was stripped and reprobed with anti-actin antibody as a loading control.

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  Immunofluorescent staining of OVCAR3 cells using anti-EZR mouse monoclonal antibody (TA807019, green, upper left; merged, upper right) or Isotype control ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

Specifications

Product Data

• Clone Name: OTI1F3
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EZR (NP_003370) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.2 kDa
• Gene Name: Homo sapiens ezrin (EZR), transcript variant 1, mRNA.
• Database Link:
  NP_003370
  Entrez Gene 22350 MouseEntrez Gene 54319 RatEntrez Gene 7430 Human
  UniProt ID P15311
• Background: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CVIL; CVL; HEL-S-105; VIL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton