Lymphocyte Activation Gene 3 (LAG3) Mouse Monoclonal Antibody [Clone ID: OTI8F6]

CAT#: CF807082

Carrier-free (BSA/glycerol-free) LAG3 mouse monoclonal antibody, clone OTI8F6 (formerly 8F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (RC220269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3 (1:2000). Positive lysates LY400828 (100ug) and LC400828 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 cell lines (Human PBMC treated with 10ug/ml PHA for 72h (Right)/untreated (Left)) lysates by using anti-LAG3 monoclonal antibody (1:150).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807082) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LAG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807082) (1:150)

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  LAG3 stable cells was stained by anti-LAG3 (TA807082, red) or IgG isotype control (TA180144, blue) and analyzed by flow. Control IgG and LAG3 were diluted 1:100

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-LAG3 antibody (TA807082) (1:100).

• Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-LAG3 antibody (TA807082). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

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Specifications

Product Data

• Clone Name: OTI8F6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 66-332 of human LAG3(NP_002277) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens lymphocyte activating 3 (LAG3), mRNA.
• Database Link:
  NP_002277
  Entrez Gene 3902 Human
  UniProt ID P18627
• Background: Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
• Synonyms: CD223

Reference Data

• Protein Families: Transmembrane