PAPSS2 Mouse Monoclonal Antibody [Clone ID: OTI1E12]

CAT#: CF807111

Carrier-free (BSA/glycerol-free) PAPSS2 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAPSS2 (RC200551, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2. Positive lysates LY417837 (100ug) and LC417837 (20ug) can be purchased separately from OriGene.

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PAPSS2-Knockout HeLa cells (KO, Cat# LC812053) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2 monoclonal antibody TA807111 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI1E12
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-247 of human PAPSS2(NP_004661) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.3 kDa
• Gene Name: Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (PAPSS2), transcript variant 1, mRNA.
• Database Link:
  NP_004661
  Entrez Gene 23972 MouseEntrez Gene 294103 RatEntrez Gene 9060 Human
  UniProt ID O95340
• Background: Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: ATPSK2; BCYM4; SK2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism, Selenoamino acid metabolism, Sulfur metabolism