TEM1 (CD248) Mouse Monoclonal Antibody [Clone ID: OTI4B8]

CAT#: CF807122

Carrier-free (BSA/glycerol-free) CD248 mouse monoclonal antibody, clone OTI4B8 (formerly 4B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD248 (RC208576, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD248. Positive lysates LY412436 (100ug) and LC412436 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4B8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-300 of human CD248(NP_065137) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.7 kDa
• Gene Name: Homo sapiens CD248 molecule (CD248), mRNA.
• Database Link:
  NP_065137
  Entrez Gene 57124 Human
  UniProt ID Q9HCU0
• Synonyms: CD164L1; TEM1

Reference Data

• Protein Families: Druggable Genome, Transmembrane