Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: OTI9B5]

CAT#: CF807138

Carrier-free (BSA/glycerol-free) ITGB1 mouse monoclonal antibody,clone OTI9B5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITGB1 (RC203818, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITGB1 (1:500). Positive lysates LY400805 (100ug) and LC400805 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-ITGB1 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ITGB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807138) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ITGB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807138) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ITGB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807138) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ITGB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807138) (1:150)

• HEK293T cells transfected with either RC203818 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-ITGB1antibody (TA807138), and then analyzed by flow cytometry (1:100).

• Flow cytometric Analysis of living Hela cells, using anti-ITGB1 antibody (TA807138), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

• Flow cytometric Analysis of living U-87MG cells, using anti-ITGB1 antibody (TA807138), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

• Flow cytometric Analysis of living 786-O cells, using anti-ITGB1 antibody (TA807138), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI9B5
• Applications: FC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ITGB1 (NP_002202) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86.1 kDa
• Gene Name: Homo sapiens integrin subunit beta 1 (ITGB1), transcript variant 1A, mRNA.
• Database Link:
  NP_002202
  Entrez Gene 16412 MouseEntrez Gene 24511 RatEntrez Gene 3688 Human
  UniProt ID P05556
• Background: Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Axon guidance, Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer