NR2C2 Mouse Monoclonal Antibody [Clone ID: OTI7E12]

CAT#: CF807249

Carrier-free (BSA/glycerol-free) NR2C2 mouse monoclonal antibody, clone OTI7E12 (formerly 7E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C2 (RC219184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR2C2 (1:2000). Positive lysates LY401137 (100ug) and LC401137 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NR2C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807249) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NR2C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807249) (1:150)

Specifications

Product Data

• Clone Name: OTI7E12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-241 of human NR2C2(NP_003289) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.2 kDa
• Gene Name: Homo sapiens nuclear receptor subfamily 2 group C member 2 (NR2C2), transcript variant 1, mRNA.
• Database Link:
  NP_003289
  Entrez Gene 22026 MouseEntrez Gene 50659 RatEntrez Gene 7182 Human
  UniProt ID P49116
• Synonyms: TAK1; TR4

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors