DR5 (TNFRSF10B) Mouse Monoclonal Antibody [Clone ID: OTI4B12]

CAT#: CF807291

Carrier-free (BSA/glycerol-free) TNFRSF10B mouse monoclonal antibody, clone OTI4B12 (formerly 4B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10B (RC219808, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF10B (1:500). Positive lysates LY407781 (100ug) and LC407781 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-TNFRSF10B monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TNFRSF10B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807291) (1:150)

Specifications

Product Data

• Clone Name: OTI4B12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 203-411 of human TNFRSF10B(NP_671716) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: Homo sapiens TNF receptor superfamily member 10b (TNFRSF10B), transcript variant 2, mRNA.
• Database Link:
  NP_671716
  Entrez Gene 8795 Human
  UniProt ID O14763
• Background: The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]
• Synonyms: CD262; DR5; KILLER; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity, p53 signaling pathway