MALT1 Mouse Monoclonal Antibody [Clone ID: OTI4C3]

CAT#: CF807326

Carrier-free (BSA/glycerol-free) MALT1 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MALT1 (RC214639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MALT1 (1:2000). Positive lysates LY402030 (100ug) and LC402030 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell lines by using anti-MALT1 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MALT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807326) (1:150)

Specifications

Product Data

• Clone Name: OTI4C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MALT1 (NP_006776) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 92.1 kDa
• Gene Name: Homo sapiens MALT1 paracaspase (MALT1), transcript variant 1, mRNA.
• Database Link:
  NP_006776
  Entrez Gene 240354 MouseEntrez Gene 307366 RatEntrez Gene 10892 Human
  UniProt ID Q9UDY8
• Background: This gene has been found to be recurrently rearranged in chromosomal translocation with two other genes - baculoviral IAP repeat-containing protein 3 (also known as apoptosis inhibitor 2) and immunoglobulin heavy chain locus - in mucosa-associated lymphoid tissue lymphomas. The protein encoded by this gene may play a role in NF-kappaB activation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: IMD12; MLT; MLT1

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: B cell receptor signaling pathway, T cell receptor signaling pathway