PR3 (PRTN3) Mouse Monoclonal Antibody [Clone ID: OTI5B9]

CAT#: CF807347

Carrier-free (BSA/glycerol-free) PRTN3 mouse monoclonal antibody, clone OTI5B9 (formerly 5B9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRTN3 (RC220949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRTN3 (1:2000). Positive lysates LY400985 (100ug) and LC400985 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5B9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-248 of human PRTN3(NP_002768) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.6 kDa
• Gene Name: Homo sapiens proteinase 3 (PRTN3), mRNA.
• Database Link:
  NP_002768
  Entrez Gene 5657 Human
  UniProt ID P24158
• Background: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-. [UniProtKB/Swiss-Prot Function]
• Synonyms: ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP-4; NP4; P29; PR-3; PR3

Reference Data

• Protein Families: Druggable Genome, Protease