Granulin (GRN) Mouse Monoclonal Antibody [Clone ID: OTI3H6]

CAT#: CF807350

Carrier-free (BSA/glycerol-free) GRN mouse monoclonal antibody, clone OTI3H6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (RC202139, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN (1:2000). Positive lysates LY419545 (100ug) and LC419545 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-GRN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807350) (1:150)

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Specifications

Product Data

• Clone Name: OTI3H6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 327-593 of human GRN(NP_002078) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.7 kDa
• Gene Name: Homo sapiens granulin precursor (GRN), mRNA.
• Database Link:
  NP_002078
  Entrez Gene 2896 Human
  UniProt ID P28799
• Background: Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]
• Synonyms: CLN11; GEP; GP88; PCDGF; PEPI; PGRN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein