PAK4 Mouse Monoclonal Antibody [Clone ID: OTI3C3]

CAT#: CF807368

Carrier-free (BSA/glycerol-free) PAK4 mouse monoclonal antibody, clone OTI3C3 (formerly 3C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (RC202302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4. Positive lysates LY417002 (100ug) and LC417002 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3C3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-308 of human PAK4(NP_005875) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.9 kDa
• Gene Name: Homo sapiens p21 (RAC1) activated kinase 4 (PAK4), transcript variant 1, mRNA.
• Database Link:
  NP_005875
  Entrez Gene 70584 MouseEntrez Gene 292756 RatEntrez Gene 10298 Human
  UniProt ID O96013
• Background: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: effector for Cdc42Hs; p21 protein (Cdc42; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; protein kinase related to S. cerevisiae STE20; Rac)-activated kinase 4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway