ECT2 Mouse Monoclonal Antibody [Clone ID: OTI2D9]
CAT#: CF807408
Carrier-free (BSA/glycerol-free) ECT2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
Formulation: Standard
Other products for "ECT2"
Specifications
Product Data | |
Clone Name | OTI2D9 |
Applications | IHC, WB |
Recommended Dilution | WB 1:2000, IHC 1:150 |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human recombinant protein fragment corresponding to amino acids 175-438 of human ECT2(NP_060568) produced in E.coli. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 99.9 kDa |
Gene Name | Homo sapiens epithelial cell transforming 2 (ECT2), transcript variant 3, mRNA. |
Database Link | |
Background | The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
Synonyms | ARHGEF31 |
Reference Data | |
Protein Families | Druggable Genome |
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