HSF2 Mouse Monoclonal Antibody [Clone ID: OTI2E11]

CAT#: CF807435

Carrier-free (BSA/glycerol-free) HSF2 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSF2 (RC210751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSF2 (1:2000). Positive lysates LY401434 (100ug) and LC401434 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-HSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807435) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-HSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807435) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-HSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807435) (1:150)

Specifications

Product Data

• Clone Name: OTI2E11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 74-324 of human HSF2(NP_004497) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.2 kDa
• Gene Name: Homo sapiens heat shock transcription factor 2 (HSF2), transcript variant 1, mRNA.
• Database Link:
  NP_004497
  Entrez Gene 15500 MouseEntrez Gene 64441 RatEntrez Gene 3298 Human
  UniProt ID Q03933
• Background: The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
• Synonyms: HSF 2; HSTF 2

Reference Data

• Protein Families: Transcription Factors