MYLIP Mouse Monoclonal Antibody [Clone ID: OTI3A2]

CAT#: CF807457

Carrier-free (BSA/glycerol-free) MYLIP mouse monoclonal antibody,clone OTI3A2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYLIP (RC201310, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYLIP (1:2000). Positive lysates LY415697 (100ug) and LC415697 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3A2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 127-385 of human MYLIP(NP_037394) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.7 kDa
• Gene Name: Homo sapiens myosin regulatory light chain interacting protein (MYLIP), mRNA.
• Database Link:
  NP_037394
  Entrez Gene 218203 MouseEntrez Gene 29116 Human
  UniProt ID Q8WY64
• Background: The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]
• Synonyms: IDOL; MIR

Reference Data

• Protein Families: Druggable Genome