IGFBP3 Mouse Monoclonal Antibody [Clone ID: OTI8E11]

CAT#: CF807472

Carrier-free (BSA/glycerol-free) IGFBP3 mouse monoclonal antibody, clone OTI8E11 (formerly 8E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3. Positive lysates LY422966 (100ug) and LC422966 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI8E11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-297 of human IGFBP3(NP_001013416) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.2 kDa
• Gene Name: Homo sapiens insulin like growth factor binding protein 3 (IGFBP3), transcript variant 1, mRNA.
• Database Link:
  NP_001013416
  Entrez Gene 3486 Human
  UniProt ID P17936
• Background: This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
• Synonyms: BP-53; IBP3

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: p53 signaling pathway