TRIB3 Mouse Monoclonal Antibody [Clone ID: OTI3F6]

CAT#: CF807482

Carrier-free (BSA/glycerol-free) TRIB3 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3. Positive lysates LY402848 (100ug) and LC402848 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807482)

Specifications

Product Data

• Clone Name: OTI3F6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-200 of human TRIB3(NP_066981) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: Homo sapiens tribbles pseudokinase 3 (TRIB3), transcript variant 1, mRNA.
• Database Link:
  NP_066981
  Entrez Gene 57761 Human
  UniProt ID Q96RU7
• Synonyms: C20orf97; NIPK; SINK; SKIP3; TRB3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors