Pellino 1 (PELI1) Mouse Monoclonal Antibody [Clone ID: OTI1D9]

CAT#: CF807506

Carrier-free (BSA/glycerol-free) PELI1 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1. Positive lysates LY412313 (100ug) and LC412313 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807506)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807506)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807506)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807506)

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Specifications

Product Data

• Clone Name: OTI1D9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-245 of human PELI1(NP_065702) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.1 kDa
• Gene Name: Homo sapiens pellino E3 ubiquitin protein ligase 1 (PELI1), mRNA.
• Database Link:
  NP_065702
  Entrez Gene 67245 MouseEntrez Gene 305549 RatEntrez Gene 57162 Human
  UniProt ID Q96FA3
• Synonyms: DKFZp686C18116; MGC50990