TRIB3 Mouse Monoclonal Antibody [Clone ID: OTI2D11]

CAT#: CF807540

Carrier-free (BSA/glycerol-free) TRIB3 mouse monoclonal antibody, clone OTI2D11 (formerly 2D11)

Product Images

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807540) (1:150)

Specifications

Product Data

• Clone Name: OTI2D11
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-200 of human TRIB3(NP_066981) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: Homo sapiens tribbles pseudokinase 3 (TRIB3), transcript variant 1, mRNA.
• Database Link:
  NP_066981
  Entrez Gene 57761 Human
  UniProt ID Q96RU7
• Background: The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
• Synonyms: C20orf97; NIPK; SINK; SKIP3; TRB3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors