S100 calcium binding protein A14 (S100A14) Mouse Monoclonal Antibody [Clone ID: OTI2G9]

CAT#: CF807603

Carrier-free (BSA/glycerol-free) S100A14 mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A14 (RC202590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A14 (1:2000). Positive lysates LY402803 (100ug) and LC402803 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2G9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human S100A14 (NP_065723) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11.5 kDa
• Gene Name: Homo sapiens S100 calcium binding protein A14 (S100A14), mRNA.
• Database Link:
  NP_065723
  Entrez Gene 57402 Human
  UniProt ID Q9HCY8
• Background: This gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). [provided by RefSeq, Dec 2011]
• Synonyms: BCMP84; S100A15