PRMT5 Mouse Monoclonal Antibody [Clone ID: OTI2H3]

CAT#: CF807621

Carrier-free (BSA/glycerol-free) PRMT5 mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807621) (1:150)

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Specifications

Product Data

• Clone Name: OTI2H3
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 238-499 of human PRMT5(NP_006100) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.5 kDa
• Gene Name: Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mRNA.
• Database Link:
  NP_006100
  Entrez Gene 27374 MouseEntrez Gene 364382 RatEntrez Gene 10419 Human
  UniProt ID O14744
• Synonyms: HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs

Reference Data

• Protein Families: Stem cell - Pluripotency